Biotransformation of Chinese Jujube with Cordyceps militaris to Enhance the Antioxidant Activity In Vitro and the Protective Effect against Ethanol-Induced Oxidative Stress in Zebrafish

Solid-state fermentation (SSF) of Chinese jujube with Cordyceps militaris was performed in the present study. The results revealed that contents total phenolic and flavonoid rice medium 50% content increased to 1.59 mg GAE/g d.w. 0.46 RE/g, respectively. changes acid composition showed a similar tendency, three forms individual compounds, namely free phenol, free/conjugated bound phenol extension time. determination DPPH, ABTS, FRAP, ferrous ion chelating capacity significantly enhanced antioxidant activity vitro, protective functions against ethanol-induced oxidative stress zebrafish were also then investigated. SSF co-treatment EtOH reduced MDA elevation activities SOD GSH-Px, along T-AOC levels dose-dependent manner adult larval zebrafish. Moreover, qRT-PCR findings demonstrated SSF-jujube capable upregulating mRNA expressions Nrf2 HO-1 downregulated NF-κB larvae. In conclusion, solid-state fermented C. an effective process, exhibited good activity, better effect stress.